Applications of Nanoparticle-Antibody Conjugates in Immunoassays and Tumor Imaging
نویسندگان
چکیده
منابع مشابه
The design and applications of nanoparticle coated microspheres in immunoassays.
Nanoparticle coated microspheres are composed of two or more materials with a core/shell structure and exhibit unique abilities that allow amplification of trace targets in immunoassays. The preparation of nanoparticle coated microspheres can be accomplished using three main strategies: (1) in-situ, (2) ex-situ, and (3) hollow sphere methods. Antibodies or biomolecules can be immobilized on the...
متن کاملEvaluation of Factors Influencing Antibody Reduction for Development of Antibody Drug Conjugates
Background: Reduction/alkylation is one of the leading strategies for the development of antibody drug conjugates (ADCs). Precise control of the reduction process would not only yield a defined number of free thiols per antibody but also result in development of more homogenous conjugates. Methods: In the present study, we investigated the effect of various dithiothreitol (DTT) concentrations, ...
متن کاملDevelopment of Antibody–Drug Conjugates Using DDS and Molecular Imaging
Antibody-drug conjugate (ADC), as a next generation of antibody therapeutics, is a combination of an antibody and a drug connected via a specialized linker. ADC has four action steps: systemic circulation, the enhanced permeability and retention (EPR) effect, penetration within the tumor tissue, and action on cells, such as through drug delivery system (DDS) drugs. An antibody with a size of ab...
متن کاملRapid flow-injection sandwich-type immunoassays of proteins using an immobilized antibody reactor and adenosine deaminase-antibody conjugates
A raptd flow-mlectron sandwtch enzyme immunoassay suitabIe for the direct determmatron of proteins m btoIogtcaI sampIes rs described.. ‘The proposed system utihzes fugfify active adenosme deammase-antibody coqugates m conlunctron with a flow-through unmunoreactor and an ammomum ion-sefectrve potentrometnc detector. After appropnate sampfe/.reagent mlectton steps, the enzyme actrvtty bound to th...
متن کاملDefined DNA/nanoparticle conjugates.
Glutathione monolayer-protected gold clusters were reacted by place exchange with 19- or 20-residue thiolated oligonucleotides. The resulting DNA/nanoparticle conjugates could be separated on the basis of the number of bound oligonucleotides by gel electrophoresis and assembled with one another by DNA-DNA hybridization. This approach overcomes previous limitations of DNA/nanoparticle synthesis ...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
ژورنال
عنوان ژورنال: The AAPS Journal
سال: 2021
ISSN: 1550-7416
DOI: 10.1208/s12248-021-00561-5